Calochortus flexuosus

  • Genus

    Calochortus

  • Family
    Liliaceae
  • Synonyms

  • Common names

    Winding mariposa lily

    Straggling mariposa lily

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Purity

  • ISTA PSD

    11

    See Details
  • AOSA PSU

    1

    See Details
  • Purity working weight (grams)

    0.3

    See Details
  • Other species (OSD)/Noxious working weight (grams)

    3

    See Details

PSU 1

Seed, with or without seed coat.
Piece of broken seed, with or without seed coat, larger than one-half the original size.

Special considerations:

  • Seeds of Cucurbitaceae and Solanaceae whether or not they are filled.
  • Pairs of Allium spp. seeds adhering together need not be separated. Refer to section 6.5 c.

TWS Notes

PSD 11

Seed, provided a portion of the testa is attached.

Piece of seed larger than one-half the original size, provided a portion of the testa is attached.

Fabaceae: cotyledons that are broken apart but held together within the testa.

Seeds and pieces of seed entirely without testa are regarded as inert matter.

Fabaceae: separated cotyledons are regarded as inert matter, irrespective of whether the radicle-plumule axis and/or more than half of the testa is attached.

TWS Notes

Purity working weight (grams)

Noxious working weight/OSD

Germination and dormancy breaking method

Media

 Agar 1%

Moistened with

 H2O

Prechill

 No

Other dormancy breaking recommendations

Check for dormancy of firm ungerminated seeds at the termination of the test, using the basic criteria indicated in the Tetrazolium section below.

Germination Temperature

 20°C

Hours light/dark

 8/16

Days in warm germ

 14

Description of Normal/Abnormal Seedlings:

Seedling conditions present (singly or in combination), which would cause the seedling to be evaluated as abnormal (not considered viable):

Cotyledons: Abnormal if less than half of normally developed cotyledon with healthy tissue remains attached.

Epicotyl: missing, however may be considered present if cotyledons are intact.

Hypocotyl: open lesions extending into conducting tissue. Shortened curled or thickened.

Root: None, weak stubby or missing primary root even with weak secondary roots.

Seedling: Albino seedling is abnormal. Primary infection in one or more essential structures. Consider infection to be secondary if seedling is well developed and normally balanced (Normal).

Modified Germination:

Test can be terminated at 14 days if the dormancy is checked with tetrazolium at the termination of the germination test.

Tetrazolium (TZ) Methodology Recommendations:

  • Imbibition method and time

    Imbibe overnight between moist media or immersed in water

  • Option(s) to prepare and cut seed for staining

    Cut next to side of embryo

  • Percentage (%) of TZ solution

    1.0

  • Time and temp of staining (hr)

    4 hours to overnight if necessary

  • List of important seed structures requiring staining:
    The embryo that stain completely red are viable.

TZ Methodology Gallery

  • Normal - slow, light staining throughout embryo